Skip to main content
. 2012 Dec 28;7(12):e52863. doi: 10.1371/journal.pone.0052863

Figure 5. Effects of Ber or/and Y on IκBα, ERK, JNK and p38 MAPK phosphorylation as well as NF-κB activation in spleen of LPS-challenged mice.

Figure 5

Spleen was removed 1 h after LPS or normal saline injection, extraction of total protein was analyzed for the presence of total and phosphorylated forms of IκBα, ERK, JNK and p38 MAPK using Western blot, cytoplasmic extracts were subjected to Western blot analysis for determining NF-κB p65 level, nuclear extracts were used to measure the NF-κB activity using NF-κB p65 transcription factor assay kit, and NF-κB activity was normalized to control. n = 5−6. *P<0.05 compared with control group; # P<0.05 compared with LPS group.