Figure 3. Brn3a expression is increased in differentiated iPS cells.

RT-qPCR of Brn3a and GAPDH mRNA was performed. (A) Gel electrophoresis of Brn3a and GAPDH expression in undifferentiated and differentiated iPS cells. The PCR products obtained after 34 cycles was run on a 3% agarose gel. (B) The relative Brn3a gene expression in undifferentiated and differentiated iPS cells (unpaired t test, p<0.0004). Data shown are mean ± SEM of three biological replicates.