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. 2012 Dec 28;7(12):e53023. doi: 10.1371/journal.pone.0053023

Figure 1. Schematic representations of the T-DNA constructs used to express human C5a in tobacco; (A) vectors used for stable transformation targeting the recombinant protein to different subcellular compartments.

Figure 1

LB: left border; RB: right border; En: −340 bp to −91 bp CaMV 35S enhancer; p35S: −90 to −1 bp CaMV 35S core promoter; t35S: CaMV 35S terminator; Ω: 5′ Tobacco mosaic virus (TMV) untranslated region; nptII: neomycin phosphotransferase gene; ER: codon optimized signal peptide from the human IL6 gene; C5a: synthetic human C5a gene, codon optimized for tobacco; SEKDEL: ER retention motif; AFVY: vacuole-targeting peptide from common bean phaseolin protein; (B) Binary ‘MagnICON’ vectors used for transient expression. TVCV-Pol: RNA-dependent RNA polymerase from Turnip vein clearing virus (TVCV); MP: TMV movement protein; TVCV-3′-NTR: TVCV 3′ untranslated region; nos: A. tumefaciens nopaline synthetase gene terminator.