Table 1. Primers used for PCR and RT-PCR analysis.
Forward-Primer | Reverse Primer | Product length | ||
Name | Sequence | Name | Sequence | |
NtC5a-fw | 5′-CCTGCTGCATTTCCTGCGAC-3′ | NtC5a-rv | 5′-ACGACACAGCACTCTGTGAAGG-3 | 166 bp |
NtC5a-fw | 5′-CCTGCTGCATTTCCTGCGAC-3′ | NtC5aER-rv | 5′-GCTCATCCTTCTCAGACAGTC-3′ | 268 bp |
NtC5a-fw | 5′-CCTGCTGCATTTCCTGCGAC-3′ | NtC5aVac-rv | 5′-GTACACGAAAGCCAGTCTG-3′ | 268 bp |
2npt-fw | 5′-TCCGGCCGCTTGGGTGGAGAG-3′ | 2npt-rv | 5′-CTGGCGCGAGCCCCTGATGCT-3′ | 450 bp |
Actin-fw | 5′-GCAACTGGGATGATATGGAGAA-3′ | Actin-rv | 5′-GTGCCTTTGCAATCCACATCTG-3′ | 850 bp |
TMV-fw | 5′-GATCCGGACGTCGAAGGTTTCGAAGG-3′ | TMV-rv | 5′-CTTGACTCTAGCTAGAGCGGCCGCTGG-3′ | 977 bp/971 bp* |
pICH29912-C5aER and pICH29912-C5aVac.