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. 2012 Dec 28;7(12):e53023. doi: 10.1371/journal.pone.0053023

Table 1. Primers used for PCR and RT-PCR analysis.

Forward-Primer Reverse Primer Product length
Name Sequence Name Sequence
NtC5a-fw 5′-CCTGCTGCATTTCCTGCGAC-3′ NtC5a-rv 5′-ACGACACAGCACTCTGTGAAGG-3 166 bp
NtC5a-fw 5′-CCTGCTGCATTTCCTGCGAC-3′ NtC5aER-rv 5′-GCTCATCCTTCTCAGACAGTC-3′ 268 bp
NtC5a-fw 5′-CCTGCTGCATTTCCTGCGAC-3′ NtC5aVac-rv 5′-GTACACGAAAGCCAGTCTG-3′ 268 bp
2npt-fw 5′-TCCGGCCGCTTGGGTGGAGAG-3′ 2npt-rv 5′-CTGGCGCGAGCCCCTGATGCT-3′ 450 bp
Actin-fw 5′-GCAACTGGGATGATATGGAGAA-3′ Actin-rv 5′-GTGCCTTTGCAATCCACATCTG-3′ 850 bp
TMV-fw 5′-GATCCGGACGTCGAAGGTTTCGAAGG-3′ TMV-rv 5′-CTTGACTCTAGCTAGAGCGGCCGCTGG-3′ 977 bp/971 bp*
*

pICH29912-C5aER and pICH29912-C5aVac.