Table 1.
Phosphorylation of NCC in different sites and conditions
| Experimental System/Model | Stimulus | Effect | Phospho-Site* | Kinase Involved | Reference No. |
|---|---|---|---|---|---|
| Xenopus oocytes | Intracellular Cl− depletion | ↑↑ | T55, T60, S73 | ? | 65 |
| Mouse | WNK4(D561A/+) knockin | ↑↑ | S73 | WNK4 | 99 |
| HEK-293 cells | Intracellular Cl− depletion | ↑↑ | T46, T55, T60, S91 | SPAK | 72 |
| Rat | Low-salt diet | ↑↑ | T55,T60, S73 | ? | 15 |
| Human urine | None | ↑ | S811 | ? | 34 |
| Xenopus oocytes/mpkDCT cells | Angiotensin II | ↑↑ | T55, T60 | WNK4-SPAK | 77 |
| Mouse | Low-K+ diet | ↑ | T55, T60, S73 | SGK1 | 88 |
| Mouse | WNK4 hypomorphic | ↓ | T55, T60, S73 | WNK4 | 64 |
| Brattleboro rats | dDAVP | ↑↑ | T55, S73 | ? | 60 |
| Brattleboro rats/Wistar rats | dDAVP | ↑↑ | T55, T60 | SPAK | 67 |
| Mouse | SPAK knockin | ↓↓ | T55, T60, S91 | SPAK | 71 |
| mpkDCT cells/mouse | Angiotensin II/aldosterone | ↑↑ | T55, T60, S73 | SPAK | 86 |
| Adrenalectomized rats | Angiotensin II/aldosterone | ↑↑ | T55, T60 | WNK4-SPAK | 89 |
| Mouse | KS-WNK1 knockout | ↑ | T45, T55, T60, S73 | WNK1/WNK4 | 36 |
| Mouse | KS-WNK1 knockout | ↑ | T60 | WNK1/WNK4 | 51 |
| In tube | Incubation with MO25 | ↑ | T45, T55, T60, S91 | SPAK/OSR1 | 25 |
| Mouse | SPAK knockout | ↓↓ | T55, T60, S73 | SPAK | 98 |
| Mouse | SPAK knockout | ↓↓ | T55, S73 | SPAK | 54 |
| mDCT cells/rats | Cyclosporine | ↑ | T55, T60, S73 | ? | 56 |
| Mouse | Tacrolimus | ↑ | T55 | WNK3/WNK4 | 38 |
| Mouse | NCC transgenic | ⇆ | T55 | ? | 55 |
| Mouse | WNK4 Knockout | ↓↓ | T60 | WNK4/SPAK | 12 |
| mpkDCT cells/mouse | Insulin | ↑ | T55, T60, S73 | WNK4/SPAK | 81 |
| Xenopus oocytes Ex vivo kidney | Insulin | ↑ | T60 | WNK3 | 14 |
| ZO obese Zucker rats | Hyperinsulinism | ↑ | T55 | WNK4 | 47 |
| Mouse | KS-OSR1 knockout | ↑ | T55 | OSR1/SPAK | 50 |
| Mouse | Isoproterenol-salt-sensitive hypertension | ↑ | T55, S73 | WNK4 | 58 |
| In tube | WNK4/Ca2+ | ↑↑ | WNK4 | 61 | |
| Xenopus oocytes | WNK3 | ↑↑ | T60 | WNK3/SPAK | 66 |
| Rats | Large-scale proteomics | ↑ | S124 | ? | 24 |
| Brattleboro rats | dDAVP | ↑ | S124 | ? | 74 |
*
Numbers correspond to human Na-Cl cotransport (NCC) sequence. DCT, distal convoluted tubule; WNK, with no lysine kinase; SPAK, Ste20-related proline/alanine-rich kinase.