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. 2012 Dec 28;7(12):e52677. doi: 10.1371/journal.pone.0052677

Figure 1. Threshold cycle values (Ct) of four housekeeping genes (18s rRNA, GAPDH, β-actin, and EF1-α) determined by qPCR from (A) testis samples of wild broodstock from different locations (Andaman sea and Gulf of Thailand) and domesticated shrimp with different growth stages (18-, 14-, 10-, and 4-month-old domesticated shrimp, DS), (B) ovary samples from wild broodstock with different ovarian maturation stages (Stages I–IV), (C) ovary samples of domesticated shrimp from different growth stages (18-, 14-, 10-, and 4-month-old domesticated shrimp, DS), and (D) ovary samples from 14-month-old domesticated broodstock before and after eyestalk ablation for 1, 4 and 7 days.

Figure 1

Different letters above the bars signify statistical differences.