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. 2012 Sep 26;303(11):F1545–F1554. doi: 10.1152/ajprenal.00400.2012

Fig. 1.

Fig. 1.

Reporter mRNAs and MS2 fusion proteins used in the recruitment assay. A: nucleotide sequence and secondary structure of an MS2 stem-loop. B: pβG-MS2 plasmid contains a tetracycline-responsive promoter element (TRE), the complete coding region for rabbit β-globin (βG), and a 3′-UTR that contains 6 copies of the MS2 stem-loop sequence (6 × MS2). The pβG-PCK2-MS2 plasmid also encodes the PCK-2 sequence that constitutes the 5′-half of the 3′-UTR of rat phosphoenolpyruvate carboxykinase (PEPCK) mRNA. C: MS2 coat protein (MS2cp) fusion contains an NH2-terminal FLAG epitope that is in-frame with the MS2cp sequence. The remaining fusion proteins also contain a hydrophilic linker sequence followed by the mouse human antigen R (HuR) or human p40-AUF1 sequence.