Fig. 8.
Two-dimensional gel analysis of the effect of pH on phosphorylation of AUF1. Confluent cultures of LLC-PK1-F+-9C cells were treated with normal or acidic medium for 24 h and then lysed with urea and thiourea. The extracts were resolved by 2-dimensional gel electrophoresis using isoelectric focusing strips that ranged from pH 6 to 11. The resulting blots were probed with anti-AUF1 antibody. Resolution of AUF1 isoforms in cells treated with normal (A) or acidic (B) medium before (−) or after (+) treatment with protein phosphatase.