Fig. 3.

Effect of hypertonic stress on protein synthesis and gpdh-1 expression. A: time course of changes in gpdh-1 mRNA levels and relative [³⁵S]methionine incorporation into total protein in worms exposed to 200 mM NaCl. Hypertonic stress inhibits [³⁵S]methionine incorporation and increases gpdh-1 expression. [³⁵S]methionine incorporation and mRNA levels are expressed relative to worms at time 0. Values for gpdh-1 mRNA levels are means ± SE (n = 3 for each time point). *P < 0.04, **P < 0.01, compared with time 0. Data on [³⁵S]methionine incorporation are replotted from Burkewitz et al. (6). Values are means ± SE (n = 5–8 for each time point). All values are P < 0.001, compared with time 0. B: effect of α-amanitin on gpdh-1 mRNA levels. Worms were exposed for 1 h to 51, 200, or 400 mM NaCl agar plates with or without 200 μg/ml α-amanitin. mRNA levels are expressed relative to worms exposed to 51 mM NaCl without α-amanitin. gpdh-1 expression in α-amanitin treated 200 and 400 mM NaCl worms was not significantly (P > 0.07) increased above that of untreated worms exposed to 51 mM NaCl. Values are means ± SE (n = 3).