Table 2.

Primers and restriction sites used for cloning the opossum kidney claudins and mouse claudin-2

	Forward Primer	Reverse Primer
Claudin-1	5′-ATG GCC AAC GCG TTG-3′	5′-TCA CAC ATA GTC CTT TCC ACT GGA GG-3′
Claudin-2	5′-CGC GGA TCC GCC ACC ATG GCC TCC CTT GGC GTT-3′ (BamHI site)	5′-CCG GAA TTC TCA CGC ATA GTC AGG AAC ATC GTA TGG GTA CAC ATA CCC AGT CAG GCT G-3′ (EcoRI site)
Claudin-4	5′-CGC GGA TCC GCC ACC ATG GGG TCC ATG GGG CTCC-3′ (BamHI site)	5′-CCG GAA TTC TCA CGC ATA GTC AGG AAC ATC GTA TGG GTA CAC GTA GTT GCT GGT AGG GGC-3′ (EcoRI site)
Claudin-6	5′-ATG GCT TCT GCC GGC CTC C-3′	5′-TTA TAC ATA ATT CTT GGC CTG GTA CTC AG-3′
Claudin-9	5′-CGG GGT ACC GCC ACC atg GCT TCA GCT GGG CTG G-3′ (KpnI site)	5′-GGA ATT CTC ACG CAT AGT CAG GAA CAT CGT ATG GGT ACA CAT AAT CCC GTT TGT CCA GG-3′ (EcoRI site)
Claudin-11	5′-CGC GGA TCC atg GTT GCC ACT TGC CTG C-3′ (BamHI site)	5′-CCG GAA TTC TCA CGC ATA GTC AGG AAC ATC GTA TGG GTA TAC GTG GGC ACT CTT GGC G-3′ (EcoRI site)
Claudin-12	5′-CGC GGA TCC GCC ACC ATG GGT TGT CGG GAT GTC cAT GC-3′ (BamHI site)	5′-CCG GAA TTC TCA CGC ATA GTC AGG AAC ATC GTA TGG GTA GGT GGC GTG GCT CAC CAC AGG-3′ (EcoRI site)
Claudin-15	5′-ATG TCA GTT GCT GTA GAG ACA TTT GGA-3′	5′-CTA TAC ATA GGC ATT TTT CCC ATA TTT GCC-3′
Claudin-20	5′-ATG GCA TCA TCA GGT CTA CAG CTC C-3′	5′-TCA TAC ATA ATC CTT CAG GTT GTA GCC TGC-3′
GAPDH	5′-CCC AAG CTT ATG TCC AAG GTG CAC ATT AGT AGA TTT GG-3′ (HindIII site)	5′-CCG GAA TTC TCA CGC ATA GTC AGG AAC ATC GTA TGG GTA CTC CTT GGT GGC CAT GTA CG-3′ (EcoRI site)

Primers used for amplification and cloning of each claudin expressed in the Didelphis virginiana opossum cDNA library and claudin-2 from mouse kidney cDNA.