Table 3.

Opossum kidney claudin RT-PCR primers and probes

Claudin-1	F: GAATTCTATGATCCCCTGACCC
	Probe: /56-FAM/CTCCTTTGCTGTTCCTGTCCCAAGA/3IABLFQ/
	R: AGGAGTCGGGTAAGAGGTTG
Claudin-4	F: CTCCAGGTAGTGGGCATTG
	Probe: /56-FAM/CCTTCATCGGCAGCAACATCGTG/3IABLFQ/
	R: ACACAGTTCATCCACAGGC
Claudin-6	F: CGTCTTGTACTGACTTCTGGG
	Probe: /56-FAM/TGCTGGACTGCTCATGCGATCA/3IABkFQ/
	R: CAACTCCCGTTTCTGGACTC
Claudin-9	F: GTGTGGAAGATGAGGTGGC
	Probe: /56-FAM/AGGACCAGGATCCCAGAGATGAGG/3IABkFQ/
	R: CACCAGAGGATTGTAGAAGTCC
Claudin-11	F: AGATTGTGTCATGGCTACGAG
	Probe: /56-FAM/AGGTTATGTCCAGGCTTGCCGAG/3IABkFQ/
	R: ACAGTCAAGAGCAGGAAGATG
Claudin-12	F: TGCTGTTCTTGTGGTACTGTG
	Probe: /56-FAM/TCTGGCCGAGTAGGGCTGAGAATA/3IABLFQ/
	R: ACAGGGATGTCTATCTCGATGG
Claudin-15	F: CCACCTCGACCATCTTTGAG
	Probe: /56FAM/TCCTTCCATGCTTGCCCTGTCTG/3IABLFQ/
	R: ATAGCTGTGATCATGAGTGCC
Claudin-20	F: GGTAAATGCAAATGTGGGCTC
	Probe: /56FAM/ATGGTACAGCACCGGGATGTTC AG/3IABkFQ/
	R: CACATACACAGGAAGGGCTAG

Successful probes and primers used in the quantitative real-time PCR experiments with claudins1, -4, -6, -9, -11, -12, -15, and -20 in opossum kidney (OK) cells.

R, reverse; F, forward.