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. 2012 Dec 28;7(12):e53074. doi: 10.1371/journal.pone.0053074

Figure 5. Notch ligand ICDs do not affect sprouting angiogenesis.

Figure 5

NOTCH1-ICD or ligand-ICD expressing HUVEC were cultured as spheroids in collagen and treated with 25 ng/ml VEGF, FGF-2 or PBS as control. The cumulative length of all sprouts was measured. Data are presented as mean cumulative sprout length per spheroid +SD. n = 3 independent experiments with 10 spheroids per condition. (A,B) Expression of Citrine-tagged ligand-ICDs or such without this tag did not significantly alter sprouting behavior. (C) The average sprout length was slightly but not significantly (p>0.05 in all cases) reduced after ligand-ICD expression. The average number of capillary sprouts was not altered. (D) NOTCH1-ICD expression prevents VEGF and FGF2-induced sprouting angiogenesis. However, this was not significantly affected by co-expression of ligand-ICDs. *, p<0.05. n.s., not significant.