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. Author manuscript; available in PMC: 2014 Jan 4.
Published in final edited form as: FEBS Lett. 2012 Nov 21;587(1):60–66. doi: 10.1016/j.febslet.2012.10.051

Fig. 3.

Fig. 3

Examination of potential Cin85 phospho-RXXS/T kinases. (A) HEK 293 cells were transfected with wildtype Cin85 (or co-transfected with myristoylated-Akt-HA as indicated). Cells were starved of serum for 12 hours, left untreated or treated with 100nM EGF or PMA for thirty minutes as indicated. Whole cell extracts were directly subjected to immunoblotting or first to anti-Flag immunoprecipitation as indicated. (B) HEK 293 cells were treated as in A except following serum starvation, cells were treated with or without the CamKII inhibitor CK59 (500 nM for 30 minutes).