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. Author manuscript; available in PMC: 2014 Jan 4.
Published in final edited form as: FEBS Lett. 2012 Nov 21;587(1):60–66. doi: 10.1016/j.febslet.2012.10.051

Fig. 4.

Fig. 4

A Cin85 Ser587Asp phosphomimetic exhibits dramatically reduced binding to Dab1 but retains its binding to CapZ. (A) HEK 293 cells co-transfected with the indicated Flag-Cin85 constructs and FKBP-Dab1 were lysed and extracts were either directly subjected to SDS-PAGE and immunoblotting with the indicated antibodies or were first subjected to anti-Flag immunoprecipitations. (B) Schematic depicting the domain structure of Cin85 highlights the amino acids critical (underlined) for the binding of Cin85 to CapZ. (C) Cin85 Pro492Ala is dramatically impaired in its ability to bind CapZ. HEK 293 cells were transfected with the indicated Flag-Cin85 constructs and cell extracts were subjected directly to immunoblotting or first to anti-Flag immunoprecipitations as indicated.