Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Alcohol. 2012 Jun 12;46(6):585–593. doi: 10.1016/j.alcohol.2012.05.004

Figure 1.

Figure 1

Methods for analyzing the effects of prenatal exposure to ethanol on ethanol-mediated second-order conditioning, as conducted on Experiment 1. Phase 1, first-order conditioning, postnatal day (PD) 14: Pups were removed from the maternal cage, cannulated and then briefly (10 min) habituated to the experimental context. Paired pups were then given ethanol (0.5, 1.0 or 2.0 g/kg), and stimulated with a conditioned stimulus (CS1) consisting of intraoral pulses of water. CS1 delivery occurred 5–20 min after ethanol. Unpaired pups were given ethanol administration 120 minutes after termination of CS1. Phase 2, second-order conditioning, postnatal day 15: Animals were stimulated with water pulses while placed in a sandpaper-lined compartment (CS2). Phase 3, tactile preference test, postnatal day 15: Time spent on sandpaper was recorded during a 5 min preference test. Experiment 2 employed similar procedures. In Experiment 2, however, CS1 delivery occurred 30–45 min after administration of 2.0 or 3.0 g/kg ethanol. The figure and legend were adapted with permission from Pautassi et al., 2011.