Skip to main content
. 2012 Dec 5;32(49):17540–17553. doi: 10.1523/JNEUROSCI.3012-12.2012

Figure 1.

Figure 1.

Loss of Caspase-6 partially protects against degeneration induced by NGF withdrawal. A, Wild-type (WT) and Caspase-6-knockout (KO) DRGs were cultured in Campenot chambers for 7 days in 50 ng/ml NGF and subsequently deprived of NGF for 36 and 60 h by washing out NGF and adding a function blocking anti-NGF antibody. Representative images of three rows of axons per chamber, per condition, in the presence or absence of NGF at 36 h. B, Axon degeneration was quantified as the percent of total axons with fragmented staining of βIII-Tubulin (TuJ1) across all wells of the chamber. To exclude the possibility that loss of Caspase-6 (or other mutations used in this study) affects the number of axons that traverse the grease barrier, we quantified the number of axons in the control (+NGF) side of each chamber and found no significant difference (data not shown). Each chamber derived from a unique embryo. n = 11 chambers per genotype; *p = 0.0130 at 36 h time point, Student's t test.