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. 2012 Oct 5;303(12):H1459–H1468. doi: 10.1152/ajpheart.00401.2012

Fig. 7.

Fig. 7.

Expression and activity of MMP-2 in cardiac fibroblasts. A and B: cardiac fibroblasts were pretreated with UB (10 μg/ml) for 30 min, followed by treatment with ISO (10 μM) for 48 h. A: cell lysates (20 μg) were analyzed by Western blot using anti-MMP-2 antibodies. *P < 0.05 vs. CTL; #P < 0.05 vs. ISO; n = 11. B: concentrated conditioned media (2 μg) were analyzed by gelatin in-gel zymography. *P < 0.01 vs. CTL; #P < 0.05 vs. ISO; n = 3. C: cells were pretreated with neutralizing anti-UB antibody (Ab) for 30 min, followed by treatment with UB for 30 min. The cells were then treated with ISO for 48 h. Cell lysates (20 μg) were analyzed by Western blot using anti-MMP-2 antibodies. *P < 0.05 vs. CTL; $P < 0.05 vs. UB + ISO; n = 3. D: cells were pretreated with recombinant UB (rUB; 10 or 100 μg/ml) for 30 min, followed by treatment with ISO for 48 h. Cell lysates (20 μg) were analyzed by Western blot using anti-MMP-2 antibodies. *P < 0.05 vs. CTL; #P < 0.05 vs. ISO; n = 3. Actin immunostaining indicates protein loading.