Fig. 4.
A: PDE5 protein detected as an intense immunoreactive band at 95 kDa in porcine coronary artery homogenates from four different animals (lanes 1–4). B and C: effect of Mel on PDE5 phosphorylation in the presence and absence of the selective MT2 receptor antagonist 4P-PDOT (10−7 M, n = 6). C shows the immunodensity of phospho-PDE5 normalized to the corresponding PDE5 protein band immunodensity. *Statistically significant difference between groups (P < 0.05).
