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Albumin permeability of cells increases with increasing population doublings. Age was defined as population doublings since passage 1. The permeability of hCB-ECs from passage 1 isolation increased with increasing population doublings. The permeability approached that of HAECs. n = 3. *P < 0.05 compared with hCB-EC (<31 doublings); #P < 0.01 compared with hCB-EC (<31 doublings).
