Fig. 10.

Ethanol does not impair STAT5B dephosphorylation or nuclear exit. Cells were treated in the absence (A, C, E, G, and I) or presence of 50 mM ethanol for 72 h (B, D, F, H, and J) at 37°C. In the continued presence of ethanol, cells were incubated with 50 nM GH for 30 min. Prewarmed medium was then added to dilute the ligand 40-fold, thereby eliminating its ability to activate receptors, and cells were incubated for up to an additional 30 min at 37°C. Cells were fixed and immunolabeled for phosphorylated STAT5B. ∗, Selected bile canaliculi. Scale bar, 10 μm.