Fig. 4.

Ethanol exposure inhibits ligand-stimulated translocation of STAT5B, but not Smad2/3. A: control (a–c) and ethanol-treated (d–f) cells were incubated with 500 pM TGF-β for 0, 10, and 30 min. Cells were fixed and immunolabeled for Smad2/3. B: control (a–c) and ethanol-treated (d–f) cells were incubated with 50 nM GH for 2, 5, and 15 min. Cells were fixed and immunolabeled for STAT5B. ∗, Selected bile canaliculi. Scale bar, 10 μm. C: ratio of nuclear to cytoplasmic fluorescence intensities was calculated for Smad2/3 (left) and STAT5B (right). Values are means ± SE from ≥3 independent experiments. *P ≤ 0.05, **P ≤ 0.01.