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. 2012 Oct 11;303(12):G1322–G1334. doi: 10.1152/ajpgi.00325.2012

Fig. 5.

Fig. 5.

Detection of KCa1.1α in colonic epithelial cells. Protein isolated from the membrane fraction of distal colonic epithelial cells was immunoblotted with an antibody against the KCa1.1 protein (Kcnma1) NeuroMab clone L6/60. An immunoreactive band consistent with KCa1.1 occurred at ∼100 kDa (arrowhead) using this monoclonal antibody. Use of the secondary antibody alone eliminated all bands (data not shown), indicating that the primary antibody was necessary for the observed result.