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Distribution of KCa1.1α in the colonic epithelium. RT-PCR of colonic crypt mRNA amplified a 357 base pair KCa1.1 product for the segment from exon-16 to exon-20. Samples included 9 crypts, 7 crypts, mucosal piece containing 6 crypts, and surface epithelium after complete removal of 10 crypts from a mucosal piece. GAPDH products indicated isolation of RNA from each sample.
