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. 2012 Oct 11;303(12):G1322–G1334. doi: 10.1152/ajpgi.00325.2012

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Copyright © 2012 the American Physiological Society

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Fig. 9. — K_Ca1.1α localization in the colonic epithelium. K_Ca1.1α was detected in distal colonic mucosa by immunoreactivity to 3 antibodies (K_Ca1.1α^IR; green; b). Actin detected with labeled phalloidin revealed the morphology of the epithelium (red; c) for cellular localization of K_Ca1.1α^IR (a). Prominent K_Ca1.1α^IR was apparent in surface cells along the lateral cell margins (arrowhead) and the brush-border membrane (arrow). Labeling was similar with each antibody, anti-K_Ca1.1α-L6/60 (A), anti-K_Ca1.1α-H300 (B), and anti-K_Ca1.1α-QEDRL (C). Scale bars, 5 μm. Crypt cross-section and longitudinal profiles are shown (D–I). Prominent K_Ca1.1α^IR was apparent in crypt cells along the lateral cell margins (arrowhead). Apical poles also exhibited punctate K_Ca1.1α^IR (arrow). Labeling was similar with anti-K_Ca1.1α-L6/60 (D and E) and anti-K_Ca1.1α-H300 (F and G) antibodies. Anti-K_Ca1.1α-QEDRL (H and I) exhibited K_Ca1.1α^IR only along the lateral margins at the basal end of the crypt cells (arrowhead); luminal margin is indicated by an arrow. Scale bars, 10 μm. Use of the secondary antibody alone led to loss of labeling (data not shown), indicating that the primary antibodies were necessary for the labeling observed.

Fig. 9. — K_Ca1.1α localization in the colonic epithelium. K_Ca1.1α was detected in distal colonic mucosa by immunoreactivity to 3 antibodies (K_Ca1.1α^IR; green; b). Actin detected with labeled phalloidin revealed the morphology of the epithelium (red; c) for cellular localization of K_Ca1.1α^IR (a). Prominent K_Ca1.1α^IR was apparent in surface cells along the lateral cell margins (arrowhead) and the brush-border membrane (arrow). Labeling was similar with each antibody, anti-K_Ca1.1α-L6/60 (A), anti-K_Ca1.1α-H300 (B), and anti-K_Ca1.1α-QEDRL (C). Scale bars, 5 μm. Crypt cross-section and longitudinal profiles are shown (D–I). Prominent K_Ca1.1α^IR was apparent in crypt cells along the lateral cell margins (arrowhead). Apical poles also exhibited punctate K_Ca1.1α^IR (arrow). Labeling was similar with anti-K_Ca1.1α-L6/60 (D and E) and anti-K_Ca1.1α-H300 (F and G) antibodies. Anti-K_Ca1.1α-QEDRL (H and I) exhibited K_Ca1.1α^IR only along the lateral margins at the basal end of the crypt cells (arrowhead); luminal margin is indicated by an arrow. Scale bars, 10 μm. Use of the secondary antibody alone led to loss of labeling (data not shown), indicating that the primary antibodies were necessary for the labeling observed.