Table 1.
List of Antibodies Used in the Study
| Antigen | Host (isotype) | Clone | Source |
|---|---|---|---|
| Flow cytometry | |||
| Gr1 (Ly6G) | Rat (IgG2b, κ) | RB6-8C5 | eBioscience (San Diego, CA) |
| F4/80 | Rat (IgG2a, κ) | BM8 | eBioscience |
| CD11b | Rat (IgG2b, κ) | M1/70 | Biolegend |
| MHCII (I-A/I-E) | Rat (IgG2b, κ) | M5/114.15.2 | eBioscience |
| CD80 (B7-1) | Armenian Hamster (IgG) | 16-10A1 | eBioscience |
| CD86 (B7-2) | Rat (IgG2a, κ) | GL1 | eBioscience |
| Ly6C | Rat (IgG2c, κ) | HK1.4 | Biolegend |
| CD281 (TLR1) | Rat (IgG2a, κ) | eBioTR23 | eBioscience |
| CD282 (TLR2) | Rat (IgG2b, κ) | 6C2 | eBioscience |
| TLR4/MD2 complex | Rat (IgG2a, κ) | MTS510 | eBioscience |
| CD289 (TLR9) | Rat (IgG2a) | M9.D6 | eBioscience |
| TLR5 | Mouse (IgG2a, κ) | 19D759.2 | Imgenex (San Diego, CA) |
| CD16/CD32 (Fc block) | Rat (IgG2a, λ) | 93 | eBioscience |
| Tissue staining | |||
| F4/80 | Rat (IgG2b) | CI:A3-1 | AbD Serotec (Raleigh, NC) |
| ICAM-1 (CD54) | Armenian Hamster (IgG1, κ) | 3E2 | BD Pharmingen (Franklin Lakes, NJ) |
| CD44 (LYVE1) | Rat (IgG1) | ALY7 | eBioscience |
| Ki67 | Rabbit | SP6 | GeneTex (Irvine, CA) |