Figure 5.

Characterization of kidney injury and recovery during mice renal IR. A: Kidney tissue sections from sham, 2-day (2 D), 3-day (3 D), and 6-day (6 D) postoperative mice were processed for immunohistochemistry with Ki-67 antibody as described in Figure 4 and in Materials and Methods. Representative fields are shown. Full quantification of Ki-67 protein expression during the course of IR is presented in Figure 6. B: Assessment of BUN levels during mice renal IR. BUN was measured using a QuantiChrom BUN assay kit (Bioassay Systems, Hayward, CA) following the manufacturer’s protocol.