Figure 6.

Expression of JADE1S, JADE1L, and HBO1 in tubular epithelium during kidney injury and regeneration. A: Immunohistochemistry on mice kidney sections was performed as described in Figure 4 and in Materials and Methods. Representative fields are shown. Quantifications of these protein expressions during the entire course of IR are presented in Figure 7. B: The effect of renal IR on JADE1L expression in mice tubular epithelium by immunofluorescence assay. Kidney sections were processed for immunofluorescence assay as described in Materials and Methods with JADE1L antibody (dilution 1:300). Alexa Fluor 488–conjugated secondary antibody (Invitrogen) was used to visualize endogenous protein expression. The nonspecific faint signal in the IR sample is due to autofluorescence. C: The effect of renal IR on JADE1S protein expression in tubular epithelium of rat kidneys. Unilateral renal IR in rats causes JADE1S protein down-regulation and recapitulates effects in mice. Injured and contralateral kidneys were processed for immunohistochemistry. The tissue sections were stained with JADE1S antibody (dilution 1:2000) as described in Figure 4. Areas in blue boxes are enlarged as indicated by arrows. Note that in rat kidneys, JADE1S protein is localized predominantly to the proximal tubular epithelium.