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. Author manuscript; available in PMC: 2012 Dec 29.
Published in final edited form as: J Cereb Blood Flow Metab. 2006 Aug 16;27(5):875–893. doi: 10.1038/sj.jcbfm.9600388

Figure 1.

Figure 1

Phosphorylation of eIF2α does not correlate with selectively vulnerable neuron populations. Immunostaining for eIF2α(P) after 10 mins cardiac arrest-induced brain ischemia and 10 mins reperfusion. (A) Brainstem, at the level of the pons where the trigeminal nerve exits, −9.8 mm posterior to bregma. The stained cell bodies are motoneurons of the facial motor nucleus of VII. (B) Sagittal section through the cerebellum, 1.2 mm lateral to midline, showing intense perinuclear eIF2α(P) staining of Purkinje cells; there was also intense staining in the neurons of the deep nuclei (not shown). (C) Posterior hypothalamic nucleus, taken from same section as in panel (D). Thalamus, 3.6 mm posterior to bregma. Polymorphic principle neurons of the posterior nucleus of the thalamus showed intense perinuclear eIF2α(P) staining. (E) Striatum, 0.7 mm anterior to bregma, showed intense perinuclear eIF2α(P) staining in the medium spiny neurons of striatum. (F) Lateral cerebral cortex, 0.3 mm posterior to bregma, showed intense eIF2α(P) staining of pyramidal neurons of the olfactory piriform cortex. All scale bars are 50 μm. Immunostaining was as previously described (DeGracia et al, 1997).