Fig. 3.

HaGR9 has extracellular C-terminus and intracellular N-terminus indicated by immunofluorescence in both Sf9 and S2 cells. HaGR9 was expressed in its native form or fused with two MYC-epitope tags at either the N- or C-terminus. a Expression constructs for native HaGR9 (controls), N-terminally MYC-epitope tagged HaGR9 (MYC:HaGR9), and C-terminally MYC-epitope tagged HaGR9 (HaGR9:MYC). b, c Immunofluorescence of HaGR9 in Sf9 and S2 cells under permeabilized and unpermeabilized conditions. Green indicates the location of MYC-epitope expression directed Alexa 488 fluorescence. Red indicates cell nuclear staining by DAPI. Scale bar = 5 μm