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. Author manuscript; available in PMC: 2012 Dec 29.
Published in final edited form as: J Immunol. 2011 Feb 28;186(7):4455–4466. doi: 10.4049/jimmunol.1000833

FIGURE 1.

FIGURE 1

Effect of time and concentration of LTD4 and LTE4 on glycoconjugate secretion from cultured rat and human conjunctival goblet cells. Cultured rat conjunctival goblet cells were serum starved for 2 h before stimulation with increasing concentrations of LTD4 for 0.5 (A) (n = 5) or 2 h (B) (n= 5), or LTD4 (10−11 M) for 0–4 h (C) (n = 4). Cultured human conjunctival goblet cells were incubated with increasing concentrations of LTD4 for 0.5 h (D) (n = 3). Cultured rat conjunctival goblet cells were serum starved for 2 h before stimulation with LTE4 (10−11 M, open circles, and 10−12 M, closed circles) for 0–4 h or the cholinergic agonist carbachol (10−4 M, inverted triangle) for 2 h (E) (n = 5), or increasing concentrations of LTE4 (closed circles) for 1 h or carbachol (10−4 M, open circles) for 2 h (F) (n = 5). The amount of glycoconjugate secretion was measured by ELLA. Data are mean ± SEM. *p < 0.05 from no addition, #p < 0.01 from no addition.