Figure 4.

Env-specific cytokine secretion and B-lymphocyte enzyme-linked immuno spot (ELISPOT) assay responses. A, Peripheral blood mononuclear cells (PBMCs) from week 28 from subjects who received 5 × 10¹⁰ viral particles of the vaccine were assessed for secretion of multiple cytokines following stimulation with pooled clade A Env peptides by cytokine multiplex bead arrays. Concentrations of each cytokine are shown. Bars reflect mean responses. GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN-γ, interferon γ; IL-2, interleukin 2; IL-3, interleukin 3; IL-4, interleukin 4; IL-5, interleukin 5; IL-9, interleukin 9; IL-10, interleukin 10; IL-13, interleukin 13; MIP-1β, macrophage inflammatory protein 1β; TNF-α, tumor necrosis factor α; TNF-β, tumor necrosis factor β. B, PBMCs from week 28 from subjects who received 5 × 10¹⁰ vp of the vaccine were assessed for memory B-lymphocyte responses following stimulation with purified BaL or ConS gp140 in B-lymphocyte ELISPOT assays. Bars reflect mean responses. Average placebo assay backgrounds are shown as dotted lines.