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. Author manuscript; available in PMC: 2013 Mar 23.
Published in final edited form as: J Tissue Eng Regen Med. 2011 Sep 23;6(9):673–686. doi: 10.1002/term.470

Figure 3.

Figure 3

Impact of substrate topography on RASMC morphology at 6 and 21 days of culture and on RASMC proliferation and matrix production at 21 days of culture. Nuclear area (A), aspect ratio (B), and degree of orientation (C) were quantified from DAPI-labeled nuclei. Biochemical analysis of RASMCs density as determined from DNA content (D), collagen content determined with a hydroxyl-proline assay (E), and soluble (bottom error bar) and insoluble matrix (top error bar – standard error) elastin content/cell as determined with the Fastin® assay (F). The study was replicated, n = 6 samples/condition/replicate and n = 12 samples/condition total. * indicates statistical difference from the spin-coated films, # indicates statistical difference from day 6, and @ indicates statistical difference from the aligned mesh.