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. Author manuscript; available in PMC: 2013 Mar 23.
Published in final edited form as: J Tissue Eng Regen Med. 2011 Sep 23;6(9):673–686. doi: 10.1002/term.470

Figure 4.

Figure 4

Impact of substrate topography on RASMC phenotype and matrix production. Representative confocal images of RASMCs after 21 days on aligned meshes (A, D), random meshes (B, E, G), and spin-coated controls (C, F). The cells stained (green/red) for markers of both contractile (i.e. SM22α) and activated (i.e. thrombospondin (TSP)) SMC phenotypes. Cells cultured on meshes and controls also stained for collagen type 1 (COL 1, localized in the cell) and trace amounts of elastin (ELN). Image (G) is a representative negative immunofluorescent control that did not receive any primary antibodies. Cell nuclei were labeled with DAPI (blue).