Figure 7.

Disomic yeast strains show increased expression of the Sup35 prion reporter. (A) Single colonies of wild-type (WT) and disomic strains carrying the SUP35-R2E2 allele and a GFP construct preceded by three stop codons were inoculated into SC medium, and the percentage of fluorescent cells was determined after 0, 8, and 24 h by flow cytometry. Shown is the ratio of GFP⁺ cells after 8 and 24 h of growth to GFP⁺ cells immediately after inoculation (0 h). The mean and SEM of at least 12 single colonies are depicted. Strains, in order, are A31114, A29843, A29845, A29846, A29847, A29848, A31110, A31111, A31112, A29450, and A31113. (B) Strains in A and A22361 (no GFP control) were grown for 24 h, and total protein was extracted. GFP protein levels were analyzed by Western blot analysis. Pgk1 was used as a loading control.