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. 2012 Dec 15;26(24):2724–2736. doi: 10.1101/gad.208306.112

Figure 2.

Figure 2.

PAR mRNAs fall into three classes. (A) Examples of the expression pattern of pre-RNA, steady-state RNA, and poly(A) tail length of an mRNA belonging to each class:Cyp2a4 (class I), Hsd17b6 (class II), and Polr2h (class III). The pre-mRNA (green) and steady-state mRNA levels (blue) (mean ± SEM, n = 3 for each time point) were measured by quantitative PCR (qPCR). Poly(A) tail length (orange) was calculated from LM-PAT assays of pooled samples (n = 3 for each time point). All of the graphs are double-plotted for easier visualization of rhythmicity, and error bars are not depicted on merged graphs for simplicity. Rhythmicity was determined by Circwave version 1.4. (B) Venn diagram showing the relationship of RESS and the three classes of PAR mRNAs. (C) The comparison of mean UTR and ORF lengths between RESS and PAR mRNAs. (NS) Not significant (Student's t-test). (D) The mRNA half-lives of PAR mRNAs (mean ± SEM) measured in AML12 cells. mRNAs were randomly picked from each category (RESS, n = 26; PAR, n = 24; PAR class I, n = 11; PAR class II, n = 5; and PAR class III, n = 8). (E) In silico analysis of mRNA half-lives of PAR mRNAs (mean ± SEM) using genome-wide mRNA half-life data from Sharova et al. (2009). All of the mRNAs available from database were included (RESS, n = 500; PAR, n = 137; PAR class I, n = 16; PAR class II, n = 13; and PAR class III, n = 8). (***) P < 0.0005, (*) P < 0.05 (Student's t-test).