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. 1979 Apr;30(1):148–156. doi: 10.1128/jvi.30.1.148-156.1979

Immunological characterization of mouse mammary tumor virus p10 and its presence in mammary tumors and sera of tumor-bearing mice.

L O Arthur, D L Fine
PMCID: PMC353309  PMID: 90156

Abstract

Mouse mammary tumor virus (MMTV) p10 and gp52 were purified and used as radiolabeled antigens in sensitive radioimmunoassays. These radioimmunoassays were specific for MMTV proteins since detergent-disrupted MMTV from C3H/HeN, RIII, and GR/N mice gave complete competition, whereas C3H/HeNf liver extracts and other lysed retroviruses did not. Both gp52 and p10 are coded by the viral genome, since MMTV grown in a heterologous cell line (feline kidney cells) competed in these assays. Sera from mammary tumor-bearing mice and mammary tumors from C3H/HeN and C3H/HeNf mice competed in both the gp52 and the p10 assays. Although these radioimmunoassays detected predominantly group-specific antigenic determinants in C3H/HeN and C3H/HeNf tumor extracts, type specificity was also found with gp52. Absorption of the anti-MMTV serum with C3H/HeNf tumor extracts removed all antibodies directed against p10 and decreased the anti-gp52 titer approximately 30-fold. When this absorbed antiserum was used at limiting dilution in the gp52 radioimmunoassay, C3H/HeN tumor extracts gave complete competition, whereas no competition was found with C3H/HeNf tumor extracts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur L. O., Bauer R. F., Orme L. S., Fine D. L. Coexistence of the mouse mammary tumor virus (MMTV) major glycoprotein and natural antibodies to MMTV in sera of mammary tumor-bearing mice. Virology. 1978 Jun 15;87(2):266–275. doi: 10.1016/0042-6822(78)90132-0. [DOI] [PubMed] [Google Scholar]
  2. Arthur L. O., Fine D. L., Bentvelzen P. Oncogenicity of murine mammary tumor virus produced in tissue culture: brief communication. J Natl Cancer Inst. 1978 Feb;60(2):461–464. doi: 10.1093/jnci/60.2.461. [DOI] [PubMed] [Google Scholar]
  3. Arthur L. O., Fine D. L. Naturally occurring humoral immunity to murine mammary tumor virus (MuMTV) and MuMTV GP52 in mice with low mammary tumor incidence. Int J Cancer. 1978 Dec;22(6):734–740. doi: 10.1002/ijc.2910220616. [DOI] [PubMed] [Google Scholar]
  4. Arthur L. O., Long C. W., Smith G. H., Fine D. L. Immunological characterization of the low-molecular-weight DNA binding protein of mouse mammary tumor virus. Int J Cancer. 1978 Oct 15;22(4):433–440. doi: 10.1002/ijc.2910220411. [DOI] [PubMed] [Google Scholar]
  5. Bolognesi D. P., Luftig R., Shaper J. H. Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures. Virology. 1973 Dec;56(2):549–564. doi: 10.1016/0042-6822(73)90057-3. [DOI] [PubMed] [Google Scholar]
  6. Cardiff R. D., Puentes M. J., Teramoto Y. A., Lund J. K. Structure of the mouse mammary tumor virus: characterization of bald particles. J Virol. 1974 Nov;14(5):1293–1303. doi: 10.1128/jvi.14.5.1293-1303.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cardiff R. D., Puentes M. J., Young L. J., Smith G. H., Teramoto Y. A., Altrock B. W., Pratt T. S. Serological and biochemical characterization of the mouse mammary tumor virus with localization of p10. Virology. 1978 Mar;85(1):157–167. doi: 10.1016/0042-6822(78)90420-8. [DOI] [PubMed] [Google Scholar]
  8. Dickson C., Atterwill M. Polyproteins related to the major core protein of mouse mammary tumor virus. J Virol. 1978 Jun;26(3):660–672. doi: 10.1128/jvi.26.3.660-672.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dickson C., Puma J. P., Nandi S. Identification of a precursor protein to the major glycoproteins of mouse mammary tumor virus. J Virol. 1975 Jan;17(1):275–282. doi: 10.1128/jvi.17.1.275-282.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fine D. L., Arthur L. O., Gardner M. B. Prevalence of murine mammary tumor virus antibody and antigens in normal and tumor-bearing feral mice. J Natl Cancer Inst. 1978 Aug;61(2):485–491. [PubMed] [Google Scholar]
  11. Fine D. L., Arthur L. O., PLOWMAN J. K., Hillman E. A., Klein F. In vitro system for production of mouse mammary tumor virus. Appl Microbiol. 1974 Dec;28(6):1040–1046. doi: 10.1128/am.28.6.1040-1046.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gautsch J. W., Lerner R., Howard D., Teramoto Y. A., Schlom J. Strain-specific markers for the major structural proteins of highly oncogenic murine mammary tumor viruses by tryptic peptide analyses. J Virol. 1978 Sep;27(3):688–699. doi: 10.1128/jvi.27.3.688-699.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hendrick J. C., François C., Calberg-Bacq C. M., Colin C., Franchimont P., Gosselin L., Kozma S., Osterrieth P. M. Radioimmunoassay for protein p28 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera and breast cancer extracts. Cancer Res. 1978 Jun;38(6):1826–1831. [PubMed] [Google Scholar]
  14. Howard D. K., Colcher D., Teramoto Y. A., Young J. M., Schlom J. Characterization of mouse mammary tumor viruses propagated in heterologous cells. Cancer Res. 1977 Aug;37(8 Pt 1):2696–2704. [PubMed] [Google Scholar]
  15. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  18. Long C. W., Berzinski T. R., Gilden R. V. Immunologic studies of the low molecular weight DNA binding protein of murine oncornaviruses. Int J Cancer. 1977 Jun 15;19(6):843–850. doi: 10.1002/ijc.2910190616. [DOI] [PubMed] [Google Scholar]
  19. Lotan R., Beattie G., Hubbell W., Nicolson G. L. Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins. Biochemistry. 1977 May 3;16(9):1787–1794. doi: 10.1021/bi00628a004. [DOI] [PubMed] [Google Scholar]
  20. Owens R. B., Hackett A. J. Tissue culture studies of mouse mammary tumor cells and associated viruses. J Natl Cancer Inst. 1972 Nov;49(5):1321–1332. [PubMed] [Google Scholar]
  21. Parks W. P., Howk R. S., Scolnick E. M., Oroszlan S., Gilden R. V. Immunochemical characterization of two major polypeptides from murine mammary tumor virus. J Virol. 1974 Jun;13(6):1200–1210. doi: 10.1128/jvi.13.6.1200-1210.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Parks W. P., Scolnick E. M. Murine mammary tumor cell clones with varying degrees of virus expression. Virology. 1973 Sep;55(1):163–173. doi: 10.1016/s0042-6822(73)81018-9. [DOI] [PubMed] [Google Scholar]
  23. Racevskis J., Sarkar N. H. Synthesis and processing of precursor polypeptides to murine mammary tumor virus structural proteins. J Virol. 1978 Jan;25(1):374–383. doi: 10.1128/jvi.25.1.374-383.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ritzi E., Martin D. S., Stolfi R. L., Spiegelman S. Plasma levels of a viral protein as a diagnostic signal for the presence of mammary tumor: the effect of tumor removal. J Exp Med. 1977 Apr 1;145(4):999–1013. doi: 10.1084/jem.145.4.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ritzi E., Martin D. S., Stolfi R. L., Spiegelman S. Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4190–4194. doi: 10.1073/pnas.73.11.4190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sarkar N. H., Taraschi N. E., Pomenti A. A., Dion A. S. Polypeptides of the mouse mammary tumor virus. II. Identification of two major glycoproteins with the viral structure. Virology. 1976 Feb;69(2):677–690. doi: 10.1016/0042-6822(76)90496-7. [DOI] [PubMed] [Google Scholar]
  27. Schochetman G., Long C. W., Oroszlan S., Arthur L., Fine D. L. Isolation of separate precursor polypeptides for the mouse mammary tumor virus glycoproteins and nonglycoproteins. Virology. 1978 Mar;85(1):168–174. doi: 10.1016/0042-6822(78)90421-x. [DOI] [PubMed] [Google Scholar]
  28. Sheffield J. B., Daly T., Dion A. S., Taraschi N. Procedures for radioimmunoassay of the mouse mammary tumor virus. Cancer Res. 1977 May;37(5):1480–1485. [PubMed] [Google Scholar]
  29. Teramoto Y. A., Cardiff R. D., Lund J. K. The structure of the mouse mammary tumor virus: isolation and characterization of the core. Virology. 1977 Mar;77(1):135–148. doi: 10.1016/0042-6822(77)90413-5. [DOI] [PubMed] [Google Scholar]
  30. Teramoto Y. A., Kufe D., Schlom J. Multiple antigenic determinants on the major surface glycoprotein of murine mammary tumor viruses. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3564–3568. doi: 10.1073/pnas.74.8.3564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Teramoto Y. A., Kufe D., Schlom J. Type-specific antigenic determinants on the major external glycoprotein of high- and low-oncogenic murine mammary tumor viruses. J Virol. 1977 Nov;24(2):525–533. doi: 10.1128/jvi.24.2.525-533.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Teramoto Y. A., Schlom J. Radioimmunoassays that demonstrate type-specific and group-specific antigenic reactivities for the major internal structural protein of murine mammary tumor viruses. Cancer Res. 1978 Jul;38(7):1990–1995. doi: 10.1203/00006450-199501000-00002. [DOI] [PubMed] [Google Scholar]
  33. Yang J., Tang R., Nandi S. Identification of the mammary tumor virus envelope glycoprotein (gp52) on mouse mammary epithelial cell surface. Biochem Biophys Res Commun. 1977 Jun 20;76(4):1044–1050. doi: 10.1016/0006-291x(77)90961-5. [DOI] [PubMed] [Google Scholar]
  34. Zangerie P. F., Calberg-Bacq C. M., Colin C., Franchimont P., François C., Gosselin L., Kozma S., Osterrieth P. M. Radioimmunoassay for glycoprotein gp47 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera. Cancer Res. 1977 Dec;37(12):4326–4331. [PubMed] [Google Scholar]

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