Table 2.
Comparison of antirestriction activity of cloned fragments and deletion and insertion mutants of the transposon Tn5053
| Plasmid | Coefficient of restriction (K)* | Restriction relief (R)† |
|---|---|---|
| pUC19 (control) | 1.0 × 10−5‡ | 1 |
| pKLH53.1 | 1.1 × 10−3 | 110 |
| pKLH53.1tniA | 1.0 × 10−3 | 100 |
| pKLH53.1tniB2 | 9.5 × 10−4 | 95 |
| pKLH53.1tniQ2 | 1.2 × 10−3 | 120 |
| pKLH53.1tniQ1 | 9.6 × 10−4 | 96 |
| pTLΔHindIII | 1.0 × 10−5 | 1 |
| pTLHindIII-ClaI | 1.0 × 10−5 | 1 |
| pTL2.5 | 1.0 × 10−5 | 1 |
| pKLH53.2 | 1.0 × 10−5 | 1 |
| pTLORF-5 | 1.1 × 10−3 | 110 |
| pSMΔORF-5 | 1.0 × 10−5 | 1 |
| pORF-5 | 5.3 × 10−3 | 530 |
*
The coefficient of restriction (K) was determined as the ratio of the titre of phage λ.0 on strain NK114 r+m+ to the titre of the same phage on strain TG-1 r−m−.
†
The restriction relief factor R = K+/K−, where K+ is K for NK114 with a plasmid, and K− is K for NK114 without a plasmid.
‡
Mean of three independent experiments.