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. 2012 Dec 10;123(1):362–379. doi: 10.1172/JCI64052

Figure 2. Adipocyte inflammation is associated with clearance of the SMRT-GPS2 complex from the IL-6 promoter.

Figure 2

(A–E) qPCR analysis of mRNA expression of SMRT, GPS2, NCOR1, adiponectin, and IL-6 in isolated human adipocytes from subcutaneous adipose tissue of lean (n = 8) and obese (n = 12) subjects. *P < 0.05. (F–H) Gene expression correlations were analyzed using Spearman statistical test. (I) Western blot analysis of protein levels of GPS2, SMRT, and NCOR1 in isolated human mature adipocytes from subcutaneous adipose tissue of lean and obese subjects (n = 3). (J) ChIP assays were performed to measure recruitment of GPS2, SMRT, NCOR1, HDAC3, and POL2 onto the IL-6 promoter, in conjunction with histone modifications (me2H3K9, indicative of repression, and me2H3K4 and acH3, indicative of activation), in isolated human adipocytes from subcutaneous adipose tissue of lean (n = 6) and obese (n = 5) subjects. IgG served as nonspecific control antibody.