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. 2012 Dec 21;123(1):493–508. doi: 10.1172/JCI64750

Figure 6. Slug enhances growth of AR-positive PCa cells.

Figure 6

(A) Control or LN-SLUG cells were injected into the flanks of nude mice and the percentage of tumor-free mice is reported as a function of time. (B) Left: relative tumor growth of control or LN-SLUG cells is plotted as fold increase in volume relative to time of detectable tumor formation. Right: control and LN-SLUG tumors at 8 weeks after palpable tumor formation. (C) Left: Prior to sacrifice, animals were injected with BrdU for 24 hours. Tumor sections were stained for the presence of BrdU incorporation, and 3 random fields from each tumor were counted. BrdU incorporation is reported as percentage positive divided by total cell number. (D) Left: tumors were sectioned and stained for H&E, Slug, and AR expression. Right: total protein was isolated from tumors, and expression of AR and Slug is shown from 3 representative samples of each tumor type. Original magnification, ×20. Statistical analyses are representative of mean ± SEM. *P < 0.05; ***P < 0.001.