Figure 12. PGF promotes lung and LN metastasis of BCCs.
1 × 106 NTC, shPGF-1, and shPGF-2 MDA-231 subclones were implanted in the MFP of SCID mice. (A) Primary tumor volumes were determined serially. *P < 0.05 vs. NTC, 1-way ANOVA. (B) Primary tumor weights were measured at the end of the experiment. *P < 0.05 vs. NTC, 1-way ANOVA. (C) Photomicrographs of H&E-stained lung sections. Scale bar: 100 μm. (D) Metastatic foci in lung sections. At least 3 random fields were counted per section. **P < 0.005 vs. NTC, 1-way ANOVA. (E) Lung DNA was analyzed by qPCR with human HK2 primers to quantify metastatic burden. **P < 0.005 vs. NTC, 1-way ANOVA. (F) LN sections were stained with human-specific vimentin antibody. Scale bar: 0.5 mm. (G) LN metastasis was quantified by image analysis. *P < 0.05 vs. NTC, 1-way ANOVA.