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. 2012 Dec 17;123(1):189–205. doi: 10.1172/JCI64993

Figure 7. Coculture of BCCs+MSCs enhances HIF-1α expression and HIF transcriptional activity in BCCs.

Figure 7

(A) HIF-1α protein levels in EV cells, DKD cells, MSCs, EV+MSCs, and DKD+MSCs cultured at 20% or 1% O2. β-Actin was used as a loading control. (B) EV and DKD cells were cotransfected with p2.1 and pSV-Renilla and cocultured with MSCs or not for 48 hours at 20% or 1% O2. The Fluc/Rluc ratio was normalized to the value for EV cells at 20% O2. *P < 0.05, **P < 0.001 vs. 20% EV; #P < 0.05 vs. 1% EV, 1-way ANOVA. (C) NTC and shCR3-1 cells were cotransfected with p2.1 and pSV-Renilla and cocultured with MSCs or not for 48 hours at 20% or 1% O2. The Fluc/Rluc ratio was normalized to NTC cells at 20% O2 (mean ± SEM; n = 3). *P < 0.05, **P < 0.001 vs. 20% NTC; #P < 0.05 vs. 20% shCR3-1; ##P < 0.01 vs. 1% shCR3-1, 1-way ANOVA.