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. 2012 Dec 17;123(1):443–454. doi: 10.1172/JCI65831

Figure 4. Ac2-26 peptide activates Src kinase.

Figure 4

(A) Immunoblots of phospho-Src (Src p-Y418) and total Src (c-Src) expression in SK-CO15 cells stimulated with control (0.1% DMSO) and Ac2-26 (3 μM) for 15 minutes. Immunoblots are representative of 3 independent experiments. Normalized signal intensity is indicated above the blots. (B) Confocal images of ROS-sensitive hydro-Cy3 in SK-CO15 cells treated with vehicle, Ac2-26 (3 μM), or Ac2-26 (3 μM) after preincubation with PP2 and AZM. Confocal micrographs are representative of 4 independent experiments. Scale bar: 20 μm. Summarized data for hydro-Cy3 fluorescence intensity are presented in the graph (mean ± SEM,*P < 0.05 vs. control, #P < 0.05 vs. Ac2-26 n = 4).