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. Author manuscript; available in PMC: 2012 Dec 31.
Published in final edited form as: Cold Spring Harb Protoc. 2011 Feb 1;2011(2):pdb.top97. doi: 10.1101/pdb.top97

FIGURE 8.

FIGURE 8

Minimal photobleaching in vivo using microlensed spinning-disk confocal microscopy. A single optical section was imaged 1000 consecutive times in three colors—EGFP (green), ECFP (blue) propidium iodide (PI; red)—in an MMTV-PyMT;ACTB-ECFP;c-fms-EGFP mouse injected intraperitonally with PI to label dead cells. Images are shown at (A) exposure 1 and (A′) exposure 1000 Scale bar, 100 μm. (For color figure, see doi: 10.1101/pdb.top97 online at www.cshprotocols.org.)