Fig. 6.
Vinculin domains regulate stable assembly of integrin–FN nanoclusters. (A) Fluorescence microscopy images for integrin binding (green) to FN (red) adhesive zones for wild-type (WT), VH and VinT12-expressing cells on 250 nm×4 (top) and 500 nm×4 patterns (bottom). Scale bars: 1 µm. (B) Frequency maps for integrin recruitment on 250 nm×4 and 500 nm×4 patterns generated by stacking individual images. For 250 nm islands, expression of VH induced clustering of integrins to FN nanoislands, whereas VinT12 did not induce any recruitment. (C,D) Frequency histograms for pad occupancy for VH and VinT12 cells on (C) 250 nm×4 and (D) 500 nm×4 patterns.