Fig. 4.

Syndecan-1 restrains lung epithelial cell migration by activating Rap1. Western blot for active and total Rap1 in (A) B2b^shRNA.scr and B2b^shRNA.hSdc1 cells and (B) wild-type and Sdc1^−/− primary ALI cultures. (C–F) Migration of (C) B2b^shRNA.scr transduced with eGFP, (D) B2b^shRNA.scr co-transduced with eGFP and Rap1^G12V, (E) B2b^shRNA.hSdc1 cells transduced with eGFP, or (F) B2b^shRNA.hSdc1 cells co-transduced with eGFP and Rap1^G12V (supplementary material Movie 2). The white asterisk identifies the same cell at 0 h and 6 h after migration. (G) Cell migration speed was measured for all conditions. *P<0.001 by one-way ANOVA and post-test Bonferroni analysis; n = 3 independent experiments.