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. 2012 Nov 1;125(21):5221–5232. doi: 10.1242/jcs.111534

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 5. — Biochemical characterization of granules from Tpk mutant cells. WT or tpk3Δ strains were (A) grown to exponential phase (Exp) and then subjected to glucose starvation for 20 minutes (Exp-Glu 20 min) or (B) grown to stationary phase (SP) and re-fed with YPD for 10 minutes (SP+YPD). Immunoblots from granule-enriched fractions (P) and their corresponding supernatants (S). The graphs represent the relative abundance for each protein between the P and S fractions, determined by densitometric quantification of the bands. (C) P fractions from a WT strain after glucose starvation or tpk3Δ strain grown to exponential phase in the absence (−) or presence (+) of RNase A.