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. 2012 Nov 1;125(21):5221–5232. doi: 10.1242/jcs.111534

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 6. — PKA catalytic subunits are required for proper accumulation of PBs and SGs during stationary phase. (A) WT, tpk1Δ, tpk2Δ or tpk3Δ cells coexpressing Dcp2–CFP and eIF4E–RFP were grown to stationary phase (SP), refed with YPD (SP+YPD) for 10 minutes and visualized by fluorescence microscopy. The inset number in each picture indicates the total number of Dcp2–CFP or eIF4E–RFP/100 cells. (B) Polysomal profiles of the strains shown in A. Numbers represent the polysome/monosome area. (C) Cells coexpressing Dcp2–YFP, eIF4E–CFP and ENO2-MS2–RFP mRNA were incubated as described in A. Data are the number of Dcp2–YFP or eIF4E–CFP granules containing ENO2-MS2–RFP mRNA/100 cells. Values are mean ± s.d. n = 5). *P<0.0001 and ^#P<0.0001, SP versus SP+YPD. Scale bar: 5 µm.