Fig. 4.

Mesenteric arteries were incubated with the PDK-1 inhibitor OSU-03012 (OSU; 10 μM), and constriction in response to ET-1 was assessed. As with PKCδ inhibition, ET-1–mediated vasoconstriction was unaffected by PDK-1 inhibition in arteries from sham group rats (A) but constriction was attenuated in arteries from IH-exposed rats (B). (C) Constriction mediated by the PKC activator PDBu in arteries from sham group rats was similar in the absence or presence of PDK-1 inhibition but was diminished by nonselective PKC inhibition with Go6983 (1 μM) or inhibition of PKCδ with rottlerin (3 μM). *P < 0.05 versus vehicle treatment; n = 6–10/group.