Fig. 6.

Total and phosphorylated PDK-1 (58–68 kDa) was measured in mesenteric artery homogenates from sham and IH group rats. Representative images are shown in (A), and the average ratios of densitometry values of phosphorylated to total PDK-1 are shown in (B). When expressed as a ratio of total PDK-1 protein, PDK-1 phosphorylation at Ser²⁴¹ was similar between the sham and IH groups. Total PDK-1 was normalized to Coomassie Blue staining and was not altered by IH exposure (C). The images shown include tissue from four rats per group and are representative of three replicates. A.U., arbitrary units.