Fig. 2.

Prasugrel metabolites (PRAS) attenuate neutrophil transmigration and CD16 surface expression. (A) Effects of PRAS (5 μM) and the negative control (Ctr) on cell transmigration, when added directly to the cells with chemoattractant (fMLP, 10 μM; PAF, 10 nM). The number of neutrophils that transmigrated was measured after 1 hour of incubation at 37°C (n = 4, mean ± S.E.M.; *P ≤ 0.05, PRAS versus Ctr). (B) CD16 surface expression was measured following PRAS exposure (2 minutes; 5 μM) using flow cytometry (data shown as GMFI, mean ± S.E.M., n = 5; **P ≤ 0.01, PRAS versus Ctr).